Journal: Frontiers in Endocrinology

Article Title: Transcriptome Analysis Reveal Candidate Genes and Pathways Responses to Lactate Dehydrogenase Inhibition (Oxamate) in Hyperglycemic Human Renal Proximal Epithelial Tubular Cells

doi: 10.3389/fendo.2022.785605

Figure Lengend Snippet: The protein expression levels of peroxisome proliferator-activated receptor-g coactivator 1α (PGC1α), caspase 3 (CASP3), B-cell lymphoma 2 apoptosis regulator (BCL2), BCL2 associated X apoptosis regulator (BAX), beclin1 (BECN1) and microtubule-associated proteins 1A/1B light chain 3 (MAP1LC3) in HK-2 cells treated with low D-glucose (LG) with different concentrations of oxamate (LGOXA-0mM, LGOXA-20mM, LGOXA-40mM and LGOXA-80mM) and high D-glucose (HG) with different concentrations of oxamate (HGOXA-0mM, HGOXA-20mM, HGOXA-40mM and HGOXA-80mM) for 24 hours. (A) Western blot gels. (B) Relative grey values. * indicates P -value < 0.05 after student’s t -test.

Article Snippet: The primary antibodies [Anti-PGC1α (ab54481), Anti-CASP3 (9665), Anti-BCL2 (ab59348), Anti-BAX (GB11690), Anti-BECN1 (bs-1353R), Anti-MAP1LC3 (14600-1-ap) and Anti-β-actin (GB12001)] and all secondary antibodies were purchased from abcom (Cambridge, MA, USA), Proteintech (Wuhan, China), BIOSS (Boston, Massachusetts, USA) and Servicebio (Wuhan, China), respectively.

Techniques: Expressing, Western Blot

Journal: Journal of nanobiotechnology

Article Title: Natural exosome-like nanoparticles derived from ancient medicinal insect Periplaneta americana L. as a novel diabetic wound healing accelerator.

doi: 10.1186/s12951-023-01923-1

Figure Lengend Snippet: Fig. 6 (A) The expression of α-SMA, CD31, VEGF, CD68 and CD90 in the regenerated mouse skin tissue in each group on the 16th day was determined by immunohistochemical staining. (B) Western blot results of MMP-9, BECLIN-1 and p62 protein expression in wound tissue of mice at day 16. *P<0.05 versus DM(+) group

Article Snippet: According to the antibody instructions, the membranes were incubated with P62 (66184-1-Ig, 1:1000, Proteintech Group, Inc.), Beclin-1 (66665-1-Ig, 1:1000, Proteintech Group, Inc.), MMP-9 (GB12132-1, 1:1000, Servicebio) or β-Actin (GB12001, 1:2000, Servicebio).

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot

Journal: Viruses

Article Title: Reduced-Beclin1-Expressing Mice Infected with Zika-R103451 and Viral-Associated Pathology during Pregnancy

doi: 10.3390/v12060608

Figure Lengend Snippet: ZIKV infection in Becn1 +/+ and Becn1 +/− pregnant dams. ( A ) Schematic diagram illustrating Zika virus (ZIKV)-infection in timed-pregnant dams. Prior to viral infection, pregnant dams received the antibody MAR1-5A3 at 2 mg/animal via intraperitoneal (ip) route at gestational day 8 followed by subcutaneous (sc) infection with ZIKV at 10 3 plaque-forming unit (PFU) in 50 µL of PBS or mock (PBS) injection at gestational day E9. ( B ) Representative Western Blots probed with antibodies against several autophagy proteins, and B-actin was used as an internal control. Adult Becn1 +/+ and Becn1 +/− brains were removed postmortem and minced according to the Materials and Methods. ( C ) Densitometric analysis using image J indicate the levels of p62, Beclin1, ATG5, LC3-I and LC3-II in brains of adult Becn1 +/+ (black bar) and Becn1 +/− (brown bar) mice. The error bars show mean ± SEM for N = 3 animals per treatment. The data were analyzed using GraphPad Prism and two-way analysis of variance (ANOVA) followed by Tukey’s test. * p < 0.05 and ** p < 0.01 vs. Becn1 +/+ . ( D ) Weight gain, expressed in grams, was measured using an analytical balance at gestation day 0 and throughout gestation period, at 3-day intervals. ( E ) Percent survival rate in pregnant dams infected with ZIKV or mock (PBS) was calculated by dividing the total number of live animals by the number of live + dead animals X 100. ( F ) Viral RNA detected in serum collected from ZIKV-infected dams on E13. ( G ) Viral RNA detected in organs removed postmortem from ZIKV-infected dams on E17. ( D – G ) Error bars show mean ± SEM for N = 5–8 animals per treatment. The data were analyzed using GraphPad Prism and two-way ANOVA followed by Tukey’s test. * p < 0.05 vs. Becn1 +/+ . ( F , G ) Viral RNA equivalent is expressed on a log10 scale after comparison with a standard curve produced using serial 10-fold dilutions of ZIKV RNA from known quantities of infectious virus.

Article Snippet: Immunoblots were labeled with primary antibodies against Beclin1 (Catalog# NB500–249), ATG5 (Catalog# NB110-53818), LC3-B (Catalog# NB600–1384) and P62/SQSTM1 (Catalog# NBP1–48320) purchased from Novus Biologicals (Centennial, CO, USA). β-actin (Catalog# sc-47778; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used as internal control.

Techniques: Infection, Virus, Injection, Western Blot, Control, Mass Measurement, Comparison, Produced

Journal: Viruses

Article Title: Reduced-Beclin1-Expressing Mice Infected with Zika-R103451 and Viral-Associated Pathology during Pregnancy

doi: 10.3390/v12060608

Figure Lengend Snippet: Potential link between ZIKV proteins and Beclin1 protein. ( A – D ) The secretions of pro-inflammatory molecules were detected in glial supernatants exposed to 50 nM of viral proteins after 8, 24- and 96-h by ELISA. Becn1 +/+ glia (black bar) and Becn1 +/− glia (brown bar). The data were analyzed by two-way ANOVA followed by Tukey’s multiple comparison test. * p < 0.05 vs. respective media control, # p < 0.05 vs. Becn1 +/+ .

Article Snippet: Immunoblots were labeled with primary antibodies against Beclin1 (Catalog# NB500–249), ATG5 (Catalog# NB110-53818), LC3-B (Catalog# NB600–1384) and P62/SQSTM1 (Catalog# NBP1–48320) purchased from Novus Biologicals (Centennial, CO, USA). β-actin (Catalog# sc-47778; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used as internal control.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Control